A Hypothesis on How the Azolla Symbiosis Mitigates Nitrous Oxide Based on In Silico Analyses

Nitrous oxide is a long-lived greenhouse gas that exists for 114 years in the atmosphere and 298-fold more potent than carbon dioxide its global warming potential. Two recent studies showcased utility of Azolla plants lesser footprint nitrous production from urea other supplements to irrigated ecosystem, which mandates exploration since there still no clear solution paddy fields or ecosystems. Here, we propose based on evolution single cytochrome oxidase subunit II protein (WP_013192178.1) cyanobiont Trichormus azollae hypothesize be able quench oxide. First, draw attention domain candidate emerging as sensory periplasmic Y_Y_Y inferred bind Secondly, phylogeny showcasing poor bootstrap support position wider clade deviation core function. Thirdly, show NtcA protein, apical N-effecting transcription factor, can putatively promoter sequence gene coding (WP_013192178.1), suggesting function associated with heterocysts N-metabolism. Our fourth point involves string histidines at C-terminal extremity WP_013192178.1 missing all T. counterparts, such are perhaps involved forming Cu center. As fifth point, showcase unique glycine-183 lengthy linker region containing multiple glycines absent proximal Nostocales cyanobacteria, predict DNA binding residue. We mechanism action our silico analyses. In total, incomplete rapid conversion likely heterocystous an sensing/quenching bioinformatics analyses past literature, have repercussions climate change consequently, future human life.